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Habib, S. and Hasnain, S.E. (2000) Differential Activity of Two Non-hr Origins during Replication of the Baculovirus Autographa californica Nuclear Polyhedrosis Virus Genome. Journal of Virology, 74 (11). pp. 5182-5189. ISSN 0022-538X

J Virol 74 p5182.pdf

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The identification of potential baculovirus origins of replication (ori) has involved the generation and characterization of defective interfering particles that contain major genomic deletions yet retain their capability to replicate by testing the replication ability of transiently transfected plasmids carrying viral sequences in infected cells. So far, there has not been any evidence to demonstrate the actual utilization of these putative origins in Autographa californica multinucleocapsid nuclear polyhedrosis virus (AcMNPV) replication. By using the method of origin mapping by competitive PCR, we have obtained quantitative data for theori activity of the HindIII-K region and theie-1 promoter sequence in AcMNPV. We also provide evidence for differential activity of the two oriin the context of the viral genome through the replication phase of viral infection. Comparison of the number of molecules representing theHindIII-K and ie-1 origins vis-à-vis the non-ori polH region in a size-selected nascent DNA preparation revealed that the HindIII-K ori is utilized ∼14 times more efficiently than the ie-1 region during the late phase of infection. HindIII-K also remains the more active ori through the early and middle replication phases. Our results provide in vivo evidence in support of the view that AcMNPV replication involves multipleori that are activated with vastly different efficiencies during the viral infection cycle.

Item Type: Article
Subjects: Genomics
Depositing User: Dr P Divakar
Date Deposited: 29 May 2015 06:46
Last Modified: 24 Aug 2017 07:23
URI: http://cdfd.sciencecentral.in/id/eprint/106

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