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Srinivas, V.R. and Bachhawat-Sikder, K. and Habib, S. and Hasnain, S.E. and Surolia, A. (2001) Expression of winged bean basic agglutinin in Spodoptera frugiperda insect cell expression system. Bioscience Reports, 21 (3). pp. 361-367. ISSN 0144-8463

Biosci Rep 21 p361.pdf

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In this paper we report the successful expression of the winged bean basic agglutinin (WBA I) in insect cells infected with a recombinant baculovirus carrying the WBA I gene and its characterization in terms of its carbohydrate binding properties. The expressed protein appears to have a lower molecular weight than the native counterpart which is consistent with the lack of glycosylation of the former. Moreover, the expressed protein maintains its dimeric nature. Hence, a role for glycosylation in modulation of dimerization of WBA I is ruled out unlike Erythrina corallodendron (EcorL). Despite this the protein is active, with its sugar specificity unaltered.

Item Type: Article
Depositing User: Users 2 not found.
Date Deposited: 29 May 2015 11:23
Last Modified: 27 Jun 2016 09:39
URI: http://cdfd.sciencecentral.in/id/eprint/110

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