Improvement in K+-limited growth rate associated with expression of the N-terminal fragment of one subunit (KdpA) of the multisubunit Kdp transporter in Escherichia coli.

Sardesai, A.A. and Gowrishankar, J. (2001) Improvement in K+-limited growth rate associated with expression of the N-terminal fragment of one subunit (KdpA) of the multisubunit Kdp transporter in Escherichia coli. Journal of Bacteriology, 183 (11). pp. 3515-3520. ISSN 0021-9193

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Official URL: http://jb.asm.org/content/183/11/3515.long

Abstract

Mutations in any one of three genes, kdpA, -B, or -C, in Escherichia coli abolish the activity of Kdp, a multisubunit K+-ATPase that belongs to the P-type ATPase family of cation transporters. We found in this study that expression in vivo of a 135-amino-acid-long N-terminal fragment (KdpA'), less than one-quarter the length of native KdpA, was able to mediate an improvement in K+-limited growth rates in two different contexts, even in the absence of both KdpC and the ATPase subunit KdpB. The first context was when KdpA' was overexpressed in cells from a heterologous inducible promoter, and the second was when KdpA' was provided with a C-terminally altered extension (following a spontaneous genetic rearrangement). Our results suggest that KdpA' provides an incipient pathway for K+ translocation which can serve to transport K+ into the cells in response to the cytoplasmic membrane potential.

Item Type:	Article
Depositing User:	Users 2 not found.
Date Deposited:	30 May 2015 19:12
Last Modified:	10 Jul 2015 07:44
URI:	http://cdfd.sciencecentral.in/id/eprint/112

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