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Sahdev, S. and Taneja, T.K. and Mohan, M. and Sah, N.K. and Khar, A.K. and Hasnain, S.E. and Athar, M. (2003) Baculoviral p35 inhibits oxidant-induced activation of mitochondrial apoptotic pathway. Biochemical and Biophysical Research Communications, 307 (3). pp. 483-490. ISSN .0006-291X

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Abstract

In this study we report that the baculovirus p35 anti-apoptotic protein prevents cell death by quenching free radicals at a very upstream step in the apoptotic pathway. Mitochondria of activated rat peritoneal macrophages as well as Spodoptera frugiperda (Sf9) insect cells, following treatment with oxidants, H(2)O(2)/UVB irradiation, release cytochrome c followed by activation of caspase-3. Transfection of macrophages/Sf9 cells with a construct carrying the p35 gene under the CMV/HSP promoters resulted in p35 expression and consequent arrest of oxidative stress-induced apoptosis. p35 expression also inhibited cytochrome c release from the mitochondria of oxidant-exposed cells and blocked caspase-3 activation.

Item Type: Article
Depositing User: Dr P Divakar
Date Deposited: 09 Jun 2015 11:14
Last Modified: 09 Jun 2015 11:14
URI: http://cdfd.sciencecentral.in/id/eprint/161

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