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Ghosh, S. and Rasheedi, S. and Rahim, S.S. and Banerjee, S. and Choudhary, R.K. and Chakhaiyar, P. and Ehtesham, N.Z. and Mukhopadhyay, Sangita and Hasnain, S.E. (2004) Method for enhancing solubility of the expressed recombinant proteins in Escherichia coli. BioTechniques, 37 (3). 418, 420, 422-433. ISSN 0736-6205

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The production of correctly folded protein in Escherichia coli is often challenging because of aggregation of the overexpressed protein into inclusion bodies. Although a number of general and protein-specific techniques are available, their effectiveness varies widely. We report a novel method for enhancing the solubility of overexpressed proteins. Presence of a dipeptide, glycylglycine, in the range of 100 mM to 1 M in the medium was found to significantly enhance the solubility (up to 170-fold) of the expressed proteins. The method has been validated using mycobacterial proteins, resulting in improved solubilization, which were otherwise difficult to express as soluble proteins in E. coli. This method can also be used to enhance the solubility of other heterologous recombinant proteins expressed in a bacterial system.

Item Type: Article
Additional Information: [Open Access from the Publisher]
Depositing User: Dr P Divakar
Date Deposited: 30 Jun 2015 11:53
Last Modified: 14 Oct 2015 11:04
URI: http://cdfd.sciencecentral.in/id/eprint/206

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