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Kenchappa, P. and Sangwan, V.S. and Ahmed, N. and Rao, K.R. and Pathengay, A. and Mathai, A. and Mansoori, T. and Das, T.P. and Hasnain, S.E. and Sharma, S. (2005) High-resolution genotyping of Pseudomonas aeruginosa strains linked to acute post cataract surgery endophthalmitis outbreaks in India. Annals of Clinical Microbiology and Antimicrobials, 4 (1). p. 19. ISSN 1476-0711

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Abstract

Background Investigation of two independent outbreaks of post cataract surgery endophthalmitis identified the reservoir of epidemic strains of P. aeruginosa. Methods Patient isolates cultured from vitreous fluid of all the nine cases and from the peripheral devices of phacoemulsification machine were subjected to high-resolution Fluorescent Amplified Fragment Length Polymorphism (FAFLP) analysis. Results FAFLP based genotyping of the isolates confirmed nosocomial transmission. Although biochemical characterization and antibiotic susceptibility profiles grouped all the isolates together, FAFLP based genotyping revealed that, all the outbreak isolates were derived from 2 different strains, with independent origins. One group of isolates was traced to phacoprobe and the second one to the internal tubing system of the phacoemulsification machine used in cataract surgery. In silico analysis indicated possible evolution in both the clusters of P. aeruginosa isolates due to genetic polymorphisms. The polymorphisms were mapped to gene products (cell envelope, outer membrane proteins) possibly having significant role in pathogenesis. Conclusion The present study is probably the first one to apply FAFLP typing successfully to investigate outbreaks of postoperative endophthalmitis (POE) in an ophthalmic setting, which was able to identify the source, and helped to make rational decisions on sterilization procedures that halted more cases of infection in these hospitals.

Item Type: Article
Depositing User: Dr P Divakar
Date Deposited: 21 Jul 2015 07:13
Last Modified: 21 Jul 2015 07:13
URI: http://cdfd.sciencecentral.in/id/eprint/266

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