Bashyam, M.D. and Hasnain, S.E. (2007) Array-Based Comparative Genomic Hybridization. Applications in Cancer and Tuberculosis. In: Bioarrays: From Basics to Diagnostics. Humana Press Inc, Totowa, N.J., USA, pp. 107-121. ISBN 978-1-58829-476-0

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Abstract

There has been a huge increase in DNA sequence data during the past decade from various biological systems. Most notably, completion of human and several pathogen genomes has enabled us to apply several high-throughput technological innovations to understand the human disease process. This chapter deals with one such technology, i.e., array-based comparative genomic hybridization (aCGH). Genomic alterations have long been implicated in several disease processes, including cancer. Earlier techniques such as conventional karyotyping, G-banding, FISH, and so on, either suffered from a lower resolution or were prohibitively expensive for whole genome coverage. The comparative genomic hybridization technique was the first step towards whole genome profiling of genomic amplifications and deletions; however, it could at best offer a resolution of approx 10–20 Megabases (Mb). The advent of the microarray technology during the later part of the 1990s has enabled the high-resolution mapping of genomic alterations at a high resolution (< 1 Mb). The present chapter discusses the aCGH technology and its use in studying cancer and Mycobacterium tuberculosis infection.

Item Type:	Book Section
Depositing User:	Users 2 not found.
Date Deposited:	22 Jul 2015 10:06
Last Modified:	01 Dec 2017 09:35
URI:	http://cdfd.sciencecentral.in/id/eprint/281

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