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Radha Rama Devi, A. and Naushad, S.M. and Krishnaprasad, C. (2006) Evaluation of total plasma homocysteine in Indian newborns using heel-prick samples. The Indian Journal of Pediatrics, 73 (6). pp. 503-508. ISSN 0019-5456

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Objective To estimate total plasma homocysteine levels in Indian newborns by modifying the existing SBD-F based High performance liquid chromotography (HPLC) method in order to enable analysis in newborn heel-prick samples and assess the prevalence of hyperhomocysteinemia in Indian newborns who are exclusively breast-fed. Methods Reverse-phase HPLC with fluorescence detection for plasma homocysteine estimation and statistical analysis using student t-test. Results SBD-F based HPLC method was modified and Bland and Altman analysis was carried out to assess agreement between original and modified methods. The correlation co-efficient was 0.994. The limits of agreement (−5.9, 6.3) were small enough to apply new method in place of the old for heel-prick sample analysis. Total plasma homocysteine analysis was carried out on heel-prick samples of 607 randomly selected newborns (331 males and 276 females). The mean plasma homocysteine estimated by this method in Indian newborns was 6.99 (95% Cl: 6.48–7.49) with no appreciable gender effect (P=0.74). Elevated homocysteine levels were observed in 31 males and 21 females. Conclusions Modified HPLC method is validated and can be used for homocysteine analysis on newborn heel-prick samples. Using this method, the prevalence of hyperhomocysteinemia in Indian newborn is 8.6%

Item Type: Article
Depositing User: Dr P Divakar
Date Deposited: 28 Jul 2015 10:22
Last Modified: 28 Jul 2015 10:22
URI: http://cdfd.sciencecentral.in/id/eprint/303

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