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Rasheedi, S. and Ramachandran, A. and Ehtesham, N.Z. and Hasnain, S.E. (2007) Biochemical characterization of Sf9 Sp-family-like protein factors reveals interesting features. Archives of Virology, 152 (10). pp. 1819-1828. ISSN 0304-8608

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We earlier documented the involvement of novel Sp-family-like protein factors in transcription from the Autographa californica nucleopolyhedrovirus (AcNPV) polyhedrin (polh) gene promoter [Ramachandran et al(2001) J. Biol. Chem. 276: 23440–23449]. These zinc-dependent Sp-like factors bind to two putative Sp-factor-binding motifs, present within the AcSp sequence upstream of the polh promoter, with very high affinity (Kd = 2.1 × 10−12 M). Like other polh-promoter-associated host transcription factors, these Sp-like factors display tolerance to high ion concentrations up to even 3 M NaCl. An electrophoretic mobility shift assay demonstrated a probable cross-talk between the Spodoptera frugiperda (Sf9) Sp-family-like proteins and the TFIID complex. In complementary experiments, specific replacements of the Sp-factor-binding motifs with TATA-like elements resulted in expression of a luciferase reporter gene to almost the same level as that obtained with a wild-type native construct. Our results point to the possibility of the involvement of TFIID and Sf9 Sp protein interaction in transcription from the baculovirus polyhedrin promoter.

Item Type: Article
Depositing User: Dr P Divakar
Date Deposited: 14 Aug 2015 11:22
Last Modified: 14 Aug 2015 11:25
URI: http://cdfd.sciencecentral.in/id/eprint/336

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