Archak, S. and Lakshminarayanareddy, V. and Nagaraju, J. (2007) High-throughput multiplex microsatellite marker assay for detection and quantification of adulteration in Basmati rice (Oryza sativa). Electrophoresis, 28 (14). pp. 2396-2405. ISSN 0173-0835

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Abstract

Basmati rice is a very special type of aromatic rice known world-wide for its extra long grains and pleasant and distinct aroma. Traditional Basmati rice cultivars, confined to Indo-Gangetic regions of the Indian subcontinent, are often reported to be adulterated with crossbred Basmati varieties and long-grain non-Basmati varieties in the export market. At present, there is no commercial scale technology to reliably detect adulteration. We report here a CE-based multiplex microsatellite marker assay for detection as well as quantification of adulteration in Basmati rice samples. The single-tube assay multiplexes eight microsatellite loci to generate variety-specific allele profiles that can detect adulteration from 1% upwards. The protocol also incorporates a quantitative-competitive PCR-based analysis for quantification of adulteration. Accuracy of quantification has been shown to be +/-1.5%. The experiments used to develop and validate the methodology are described.

Item Type:	Article
Depositing User:	Users 2 not found.
Date Deposited:	18 Aug 2015 06:16
Last Modified:	18 Aug 2015 06:16
URI:	http://cdfd.sciencecentral.in/id/eprint/349

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