Sawhney, B. and Chopra, K. and Mishra, R. and Ranjan, Akash (2015) Identification of Plasmodium falciparum apicoplast-targeted tRNA-guanine transglycosylase and its potential inhibitors using comparative genomics, molecular modelling, docking and simulation studies. Journal of Biomolecular Structure and Dynamics, 33 (11). pp. 2404-2420. ISSN 0739-1102

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Abstract

tRNA modifications play an important role in the proper folding of tRNA and thereby determine its functionality as an adaptor molecule. Notwithstanding the centrality of this basic process in translation, a major gap in the genomics of P. falciparum is unambiguous identification of enzymes catalysing the various tRNA modifications. In this study, tRNA modifying enzymes of P. falciparum were annotated using homology based approach. Based on the presence of these identified enzymes, the modifications were compared with those of prokaryotic and eukaryotic organisms. Through sequence comparison and phylogenetic analysis, we have identified P. falciparum apicoplast tRNA-guanine 34 transglycosylase (TGT, EC: 2.4.2.29), which shows evidence of its prokaryotic origin. The docking analysis of the modeled TGT structures revealed that binding of quinazolinone derivatives is more favourable with P. falciparum apicoplast TGT as compared to human TGT. Molecular Dynamic Simulation and MM/GBSA analysis of the complex confirmed the greater binding affinity of the ligand in the binding pocket of P. falciparum TGT protein. Further, evolutionary patterning analysis identified the amino acids of P. falciparum apicoplast TGT that are under purifying selection pressure and hence can be good inhibitor targeting sites. Based on these computational studies, we suggests that P. falciparum apicoplast tRNA-guanine 34 transglycosylase can be a promising drug target.

Item Type:	Article
Uncontrolled Keywords:	tRNA modifying enzymes, tRNA-guanine transglycosylase, docking and MD simulation, evolutionary patterning
Depositing User:	Users 2 not found.
Date Deposited:	16 May 2015 18:32
Last Modified:	05 Oct 2017 07:43
URI:	http://cdfd.sciencecentral.in/id/eprint/35

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