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Gadipally, S.R. and Sarkar, A. and Nandineni, M.R. (2015) Selective enrichment of STRs for applications in forensic human identification. Electrophoresis, 36 (15). pp. 1768-1774. ISSN 0173-0835

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Abstract

Forensic human identification (HID) is currently based on determining repeat length polymorphisms located in short tandem repeat regions in the human genome. Despite the great progress made in the area of multiplex PCR-based approaches, limitations associated with challenging forensic samples such as DNA degradation, co-occurrence of inhabited microbial DNA and PCR inhibitors significantly affect the success rate of human DNA profiling. We have developed a sequence-specific pre-PCR STR enrichment method and evaluated its efficacy using DNA samples doped with various contaminants in view of its application on compromised forensic samples. This strategy has enabled us to generate complete and reproducible DNA profiles from samples doped with 5-fold excess of non-human DNA and 3-4 fold excess of various potent PCR inhibitors than that is claimed to be tolerated by some of the widely used commercial multiplex STR kits, from as little as 2 nanograms of degraded human DNA. The 'hybrid capture' based STR enrichment strategy described in this study is easily adaptable and offers a sensitive, efficient and economical approach for successful human DNA profiling from compromised and recalcitrant forensic samples that are usually encountered in mass disaster incidents and missing persons identifications. This article is protected by copyright. All rights reserved.

Item Type: Article
Uncontrolled Keywords: DNA profiling; Forensic genotyping; PCR inhibitors; STR enrichment
Depositing User: Dr P Divakar
Date Deposited: 16 May 2015 19:02
Last Modified: 26 Apr 2016 08:50
URI: http://cdfd.sciencecentral.in/id/eprint/37

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