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Werner, J.K. and Speed, T. and Bhandari, Rashna (2010) Protein Pyrophosphorylation by Diphosphoinositol Pentakisphosphate (InsP 7). Methods in Molecular Biology, 645. pp. 87-102. ISSN 1064-3745

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Diphosphoinositol polyphosphates, also known as inositol pyrophosphates, are a family of water soluble inositol phosphates that possess diphosphate or pyrophosphate moieties. In the presence of divalent cations such as Mg(2+), the "high energy" beta phosphate can be transferred from the inositol pyrophosphates, InsP(7) and InsP(8), to prephosphorylated serine residues on proteins, to form pyrophosphoserine. This chapter provides detailed methods to identify proteins that are substrates for pyrophosphorylation by InsP(7), conduct phosphorylation assays on purified protein, and detect protein pyrophosphorylation.

Item Type: Article
Uncontrolled Keywords: Inositol pyrophosphate Diphosphoinositol pentakisphosphate Pyrophosphoserine Pyrophosphorylation Phosphorylation
Depositing User: Dr P Divakar
Date Deposited: 31 Aug 2015 06:51
Last Modified: 28 Feb 2018 12:04
URI: http://cdfd.sciencecentral.in/id/eprint/415

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