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Khamrui, S. and Ranjan, Amitabh and Pani, B. and Sen, Ranjan and Sen, Udayaditya (2010) Crystallization and preliminary X-ray analysis of Psu, an inhibitor of the bacterial transcription terminator Rho. Acta Crystallographica Section F Structural Biology and Crystallization Communications, 66 (2). pp. 204-206. ISSN 1744-3091

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Abstract

Psu, a coat protein from bacteriophage P4, inhibits Rho-dependent transcription termination both in vivo and in vitro. The Psu protein is α-helical in nature and appeared to be a dimer in solution. It interacts with Rho and affects the ATP binding and RNA-dependent ATPase activity of Rho, which in turn reduces the rate of RNA release from the elongation complex. Crystals of Psu were grown in space group I422 in the presence of PEG, with unit-cell parameters a = b = 148.76, c = 63.38 Å and a calculated Matthews coefficient of 2.1 Å3 Da−1 (41.5% solvent content), assuming the presence of two molecules in the asymmetric unit. A native data set was collected to 2.3 Å resolution.

Item Type: Article
Additional Information: [Open Access from PubMed Central] http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2815694/
Depositing User: Dr P Divakar
Date Deposited: 01 Sep 2015 06:17
Last Modified: 10 Dec 2015 11:21
URI: http://cdfd.sciencecentral.in/id/eprint/424

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