[feed] Atom [feed] RSS 1.0 [feed] RSS 2.0

Muteeb, G. and Sen, Ranjan (2010) Random Mutagenesis Using a Mutator Strain. Methods in Molecular Biology, 634. pp. 411-419. ISSN 1064-3745

[img] Text
MMB 634 p411.pdf
Restricted to Repository staff only

Download (1439Kb) | Request a copy

Abstract

Random mutagenesis is a useful technique to study the functions of different gene products. Propagation of the genes cloned in plasmids through a mutator strain, like Escherichia coli XL1-red, produces randomly mutagenized plasmid libraries. This method offers a very simple and economic way of introducing random point mutations throughout the gene with a fairly high mutation rate. The whole process involves transformation and propagation of a plasmid containing the desired gene into the XL-1 red strain, isolating the mutagenized plasmid library and transforming this library into a desired strain for screening the mutant phenotype.

Item Type: Article
Depositing User: Dr P Divakar
Date Deposited: 01 Sep 2015 06:26
Last Modified: 26 Apr 2018 19:16
URI: http://cdfd.sciencecentral.in/id/eprint/425

Actions (login required)

View Item View Item