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Thiagarajan, D. and Rachana, R.D. and Khosla, Sanjeev (2011) The DNA methyltranferase Dnmt2 participates in RNA processing during cellular stress. Epigenetics, 6 (1). pp. 103-113. ISSN 1559-2294

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The strong evolutionary conservation of the DNA methyltransferase, Dnmt2, is at odds with the absence of phenotypic defects in organisms lacking Dnmt2. The cellular processes where Dnmt2 has a role to play also remain largely undiscovered. Here we show that Dnmt2 is a part of RNA processing machinery during cellular stress. In addition to interacting with proteins involved in RNA processing and cellular stress, Dnmt2 exhibits nucleo-cytoplasmic shuttling in response to cellular stress. Normally present in the nucleus, under conditions of stress, Dnmt2 relocalises to the cytoplasmic Stress Granules and RNA processing bodies. Surprisingly, for a DNA methyltransferase, knockout of which showed no phenotypic defects in several species, our results show that transient transfection of Dnmt2 in mammalian cells causes cell lethality. Interestingly, Dnmt2 overexpression altered the expression of several genes involved in viral infection. Taking into consideration its recently identified role in retrotransposon silencing, the role of Dnmt2 in stress granules could represent a primitive cellular defense mechanism against viral infection.

Item Type: Article
Depositing User: Dr P Divakar
Date Deposited: 01 Sep 2015 10:18
Last Modified: 12 May 2017 07:24
URI: http://cdfd.sciencecentral.in/id/eprint/432

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