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Kwei, K.A. and Shain, A.H. and Bair, R. and Montgomery, K. and Karikari, C.A. and van de Rijn, M. and Hidalgo, M. and Maitra, A. and Bashyam, M.D. and Pollack, J.R. (2011) SMURF1 Amplification Promotes Invasiveness in Pancreatic Cancer. PLoS ONE, 6 (8). e23924. ISSN 1932-6203

PLoS One 6 e23924.pdf

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Pancreatic cancer is a deadly disease, and new therapeutic targets are urgently needed. We previously identified DNA amplification at 7q21-q22 in pancreatic cancer cell lines. Now, by high-resolution genomic profiling of human pancreatic cancer cell lines and human tumors (engrafted in immunodeficient mice to enrich the cancer epithelial fraction), we define a 325 Kb minimal amplicon spanning SMURF1, an E3 ubiquitin ligase and known negative regulator of transforming growth factor β (TGFβ) growth inhibitory signaling. SMURF1 amplification was confirmed in primary human pancreatic cancers by fluorescence in situ hybridization (FISH), where 4 of 95 cases (4.2%) exhibited amplification. By RNA interference (RNAi), knockdown of SMURF1 in a human pancreatic cancer line with focal amplification (AsPC-1) did not alter cell growth, but led to reduced cell invasion and anchorage-independent growth. Interestingly, this effect was not mediated through altered TGFβ signaling, assayed by transcriptional reporter. Finally, overexpression of SMURF1 (but not a catalytic mutant) led to loss of contact inhibition in NIH-3T3 mouse embryo fibroblast cells. Together, these findings identify SMURF1 as an amplified oncogene driving multiple tumorigenic phenotypes in pancreatic cancer, and provide a new druggable target for molecularly directed therapy.

Item Type: Article
Depositing User: Dr P Divakar
Date Deposited: 02 Sep 2015 10:11
Last Modified: 09 Sep 2016 09:39
URI: http://cdfd.sciencecentral.in/id/eprint/447

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