Muteeb, G. and Dey, D. and Mishra, S. and Sen, Ranjan (2012) A multipronged strategy of an anti-terminator protein to overcome Rho-dependent transcription termination. Nucleic Acids Research, 40 (22). pp. 11213-11228. ISSN 0305-1048

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Abstract

One of the important role of Rho-dependent transcription termination in bacteria is to prevent gene expressions from the bacteriophage DNA. The transcription anti-termination systems of the lambdoid phages have been designed to overcome this Rho action. The anti-terminator protein N has three interacting regions, which interact with the mRNA, with the NusA and with the RNA polymerase. Here, we show that N uses all these interaction modules to overcome the Rho action. N and Rho co-occupy their overlapping binding sites on the nascent RNA (the nutR/tR1 site), and this configuration slows down the rate of ATP hydrolysis and the rate of RNA release by Rho from the elongation complex. N-RNA polymerase interaction is not too important for this Rho inactivation process near/at the nutR site. This interaction becomes essential when the elongation complex moves away from the nutR site. From the unusual NusA-dependence property of a Rho mutant E134K, a suppressor of N, we deduced that the N-NusA complex in the anti-termination machinery reduces the efficiency of Rho by removing NusA from the termination pathway. We propose that NusA-remodelling is also one of the mechanisms used by N to overcome the termination signals.

Item Type:	Article
Depositing User:	Users 2 not found.
Date Deposited:	11 Sep 2015 07:01
Last Modified:	28 Nov 2015 19:09
URI:	http://cdfd.sciencecentral.in/id/eprint/488

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