[feed] Atom [feed] RSS 1.0 [feed] RSS 2.0

Akif, M. and Akhter, Y. and Hasnain, S.E. and Mande, S.C. (2006) Crystallization and preliminary X-ray crystallographic studies ofMycobacterium tuberculosisCRP/FNR family transcription regulator. Acta Crystallographica Section F Structural Biology and Crystallization Communications, 62 (9). pp. 873-875. ISSN 1744-3091

[img]
Preview
Text
Acta Cryst F 62 p873.pdf

Download (109Kb) | Preview

Abstract

CRP/FNR family members are transcription factors that regulate the transcription of many genes in Escherichia coli and other organisms. Mycobacterium tuberculosis H37Rv contains a probable CRP/FNR homologue encoded by the open reading frame Rv3676. The deletion of this gene is known to cause growth defects in cell culture, in bone marrow-derived macrophages and in a mouse model of tuberculosis. The mycobacterial gene Rv3676 shares approximately 32% sequence identity with prototype E. coli CRP. The structure of the protein might provide insight into transcriptional regulation in the pathogen by this protein. The M. tuberculosis CRP/FNR transcription regulator was crystallized in space group P2(1)2(1)2(1), with unit-cell parameters a = 54.1, b = 84.6, c = 101.2 A. The crystal diffracted to a resolution of 2.9 A. Matthews coefficient and self-rotation function calculations reveal the presence of two monomers in the asymmetric unit.

Item Type: Article
Depositing User: Dr P Divakar
Date Deposited: 20 Oct 2015 08:34
Last Modified: 20 Oct 2015 08:34
URI: http://cdfd.sciencecentral.in/id/eprint/555

Actions (login required)

View Item View Item