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Tundup, S. and Pathak, Niteen and Ramanadham, M. and Mukhopadhyay, Sangita and Murthy, K.J.R. and Ehtesham, N.Z. and Hasnain, S.E. (2008) The Co-Operonic PE25/PPE41 Protein Complex of Mycobacterium tuberculosis Elicits Increased Humoral and Cell Mediated Immune Response. PLoS ONE, 3 (10). e3586. ISSN 1932-6203

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Abstract

Background: Many of the PE/PPE proteins are either surface localized or secreted outside and are thought to be a source of antigenic variation in the host. The exact role of these proteins are still elusive. We previously reported that the PPE41 protein induces high B cell response in TB patients. The PE/PPE genes are not randomly distributed in the genome but are organized as operons and the operon containing PE25 and PPE41 genes co-transcribe and their products interact with each other. Methodology/Principal Finding: We now describe the antigenic properties of the PE25, PPE41 and PE25/PPE41 protein complex coded by a single operon. The PPE41 and PE25/PPE41 protein complex induces significant (p,0.0001) B cell response in sera derived from TB patients and in mouse model as compared to the PE25 protein. Further, mice immunized with the PE25/PPE41 complex and PPE41 proteins showed significant (p,0.00001) proliferation of splenocyte as compared to the mice immunized with the PE25 protein and saline. Flow cytometric analysis showed 15–22% enhancement of CD8+ and CD4+ T cell populations when immunized with the PPE41 or PE25/PPE41 complex as compared to a marginal increase (8–10%) in the mice immunized with the PE25 protein. The PPE41 and PE25/PPE41 complex can also induce higher levels of IFN-c, TNF-a and IL-2 cytokines. Conclusion: While this study documents the differential immunological response to the complex of PE and PPE vis-a`-vis the individual proteins, it also highlights their potential as a candidate vaccine against tuberculosis.

Item Type: Article
Depositing User: Dr P Divakar
Date Deposited: 28 Oct 2015 11:04
Last Modified: 28 Oct 2015 11:04
URI: http://cdfd.sciencecentral.in/id/eprint/568

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