Flatt, T. and Singh, Y.T. and Mazumdar-Leighton, S. and Saikia, M. and Pant, P. and Kashung, S. and Neog, K. and Chakravorty, R. and Nair, S. and Nagaraju, J. and Babu, C.R. (2012) Genetic Variation within Native Populations of Endemic Silkmoth Antheraea assamensis (Helfer) from Northeast India Indicates Need for In Situ Conservation. PLoS ONE, 7 (11). e49972. ISSN 1932-6203

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Abstract

A. assamensis is a phytophagous Lepidoptera from Northeast India reared on host trees of Lauraceae family for its characteristic cocoon silk. Source of these cocoons are domesticated farm stocks that crash frequently and/or wild insect populations that provide new cultures. The need to reduce dependence on wild populations for cocoons necessitates assessment of genetic diversity in cultivated and wild populations. Molecular markers based on PCR of Inter-simple sequence repeats (ISSR) and simple sequence repeats (SSR) were used with four populations of wild insects and eleven populations of cultivated insects. Wild populations had high genetic diversity estimates (Hi = 0.25; HS = 0.28; HE = 0.42) and at least one population contained private alleles. Both marker systems indicated that genetic variability within populations examined was significantly high. Among cultivated populations, insects of the Upper Assam region (Hi = 0.19; HS = 0.18; HE = 0) were genetically distinct (FST = 0.38 with both marker systems) from insects of Lower Assam (Hi = 0.24; HS = 0.25; HE = 0.3). Sequencing of polymorphic amplicons suggested transposition as a mechanism for maintaining genomic diversity. Implications for conservation of native populations in the wild and preserving in-farm diversity are discussed.

Item Type:	Article
Depositing User:	Users 2 not found.
Date Deposited:	23 Nov 2015 11:08
Last Modified:	23 Nov 2015 11:08
URI:	http://cdfd.sciencecentral.in/id/eprint/670

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