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Zhang, J. and Zhang, P. and Wei, Y. and Piao, H.-L. and Wang, W. and Subba Reddy, Maddika and Wang, M. and Chen, D. and Sun, Y. and Hung, M.-C. and Chen, J. and Ma, Li (2013) Deubiquitylation and stabilization of PTEN by USP13. Nature Cell Biology, 15 (12). pp. 1486-1494. ISSN 1465-7392

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The tumour suppressor PTEN is frequently lost in human cancers. In addition to gene mutations and deletions, recent studies have revealed the importance of post-translational modifications, such as ubiquitylation, in the regulation of PTEN stability, activity and localization. However, the deubiquitylase that regulates PTEN polyubiquitylation and protein stability remains unknown. Here we screened a total of 30 deubiquitylating enzymes (DUBs) and identified five DUBs that physically associate with PTEN. One of them, USP13, stabilizes the PTEN protein through direct binding and deubiquitylation of PTEN. Loss of USP13 in breast cancer cells promotes AKT phosphorylation, cell proliferation, anchorage-independent growth, glycolysis and tumour growth through downregulation of PTEN. Conversely, overexpression of USP13 suppresses tumorigenesis and glycolysis in PTEN-positive but not PTEN-null breast cancer cells. Importantly, USP13 protein is downregulated in human breast tumours and correlates with PTEN protein levels. These findings identify USP13 as a tumour-suppressing protein that functions through deubiquitylation and stabilization of PTEN.

Item Type: Article
Depositing User: Dr P Divakar
Date Deposited: 24 Nov 2015 08:35
Last Modified: 29 Nov 2015 18:23
URI: http://cdfd.sciencecentral.in/id/eprint/674

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