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Chen, Z. and Nohata, J. and Guo, H. and Li, S. and Liu, J. and Guo, Y. and Yamamoto, K. and Kadono-Okuda, K. and Liu, C. and Arunkumar, K.P. and Nagaraju, J. and Zhang, Y. and Liu, S. and Labropoulou, V. and Swevers, L. and Tsitoura, P. and Iatrou, K. and Gopinathan, K.P. and Goldsmith, M.R. and Xia, Q. and Mita, K. (2015) Construction, complete sequence, and annotation of a BAC contig covering the silkworm chorion locus. Scientific Data, 2. p. 150062. ISSN 2052-4463

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Abstract

The silkmoth chorion was studied extensively by F.C. Kafatos’ group for almost 40 years. However, the complete structure of the chorion locus was not obtained in the genome sequence of Bombyx mori published in 2008 due to repetitive sequences, resulting in gaps and an incomplete view of the locus. To obtain the complete sequence of the chorion locus, expressed sequence tags (ESTs) derived from follicular epithelium cells were used as probes to screen a bacterial artificial chromosome (BAC) library. Seven BACs were selected to construct a contig which covered the whole chorion locus. By Sanger sequencing, we successfully obtained complete sequences of the chorion locus spanning 871,711 base pairs on chromosome 2, where we annotated 127 chorion genes. The dataset reported here will recruit more researchers to revisit one of the oldest model systems which has been used to study developmentally regulated gene expression. It also provides insights into egg development and fertilization mechanisms and is relevant to applications related to improvements in breeding procedures and transgenesis.

Item Type: Article
Depositing User: Dr P Divakar
Date Deposited: 03 Dec 2015 08:38
Last Modified: 03 Dec 2015 08:38
URI: http://cdfd.sciencecentral.in/id/eprint/685

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