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Vishalini, V. and Agrawal, Sonia and Sen, Ranjan (2016) Molecular Basis of NusG-mediated Regulation of Rho-dependent Transcription Termination in Bacteria. Journal of Biological Chemistry, 291 (43). pp. 22386-22403. ISSN 0021-9258

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Abstract

The bacterial transcription elongation factor NusG stimulates the Rho-dependent transcription termination through a direct interaction with Rho. The mechanistic basis of NusG dependence of the Rho function is not known. Here, we describe Rho mutants I168V, R221C/A, and P235H that do not require NusG for their termination function. These Rho mutants have acquired new properties, which otherwise would have been imparted by NusG. A detailed analyses revealed that they have more stable interactions at the secondary RNA binding sites of Rho, which reduced the lag in initiating its ATPase as well as the translocase activities. These more stable interactions arose from the significant spatial re-orientations of the P, Q, and R structural loops of the Rho central channel. We propose that NusG imparts similar conformational changes in the central channel of Rho, yielding faster isomerization of the open to the closed hexameric states of the latter during its RNA-loading step. This acceleration stabilizes the Rho-RNA interactions at many terminators having suboptimal rut sites, thus making Rho-NusG interactions so essential in vivo. Finally, identification of the NusG binding sites on the Rho hexamer led us to conclude that the former exerts its effect allosterically.

Item Type: Article
Depositing User: Dr P Divakar
Date Deposited: 25 Nov 2016 04:06
Last Modified: 02 Apr 2018 10:59
URI: http://cdfd.sciencecentral.in/id/eprint/757

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