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Pandey, S.S. and Singh, Prashantee and Samal, Biswajit and Verma, Raj Kumar and Chatterjee, Subhadeep (2017) Xanthoferrin Siderophore Estimation from the Cell-free Culture Supernatants of Different Xanthomonas Strains by HPLC. BIO-PROTOCOL, 7 (14). e2410. ISSN 2331-8325

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Xanthomonads can scavenge iron from the extracellular environment by secreting the siderophores, which are synthesized by the proteins encoded by xss (Xanthomonas siderophore synthesis) gene cluster. The siderophore production varies among xanthomonads in response to a limited supply of iron where Xanthomonas campestris pv. campestris (Xcc) produces less siderophores than Xanthomonas oryzae pv. oryzae (Xoo) and Xanthomonas oryzae pv. oryzicola (Xoc). Siderophore production can be measured by HPLC and with the CAS (Chrome azurol S)-agar plate assay, however HPLC is a more accurate method over CAS-agar plate assay for siderophore quantification in Xanthomonads. Here we describe how to quantify siderophores from xanthomonads using HPLC.

Item Type: Article
Depositing User: Dr P Divakar
Date Deposited: 08 Sep 2017 05:29
Last Modified: 08 Sep 2017 05:29
URI: http://cdfd.sciencecentral.in/id/eprint/792

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