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Anwar, T. and Sen, B. and Aggarwal, S. and Nath, R. and Katoch, A. and Aiyaz, M. and Trehanpati, N. and Khosla, Sanjeev and Ramakrishna, Gayatri (2018) Differentially regulated gene expression in quiescence vs. senescence and identification of ARID5A as a quiescence associated marker. Journal of Cellular Physiology, 233 (5). pp. 3695-3712. ISSN 0021-9541

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Abstract

In multicellular organisms majority of the cells remain in a non-dividing states of either fully differentiated or quiescence (reversible) or senescence (irreversible) conditions. In the present study, gene expression signatures unique to quiescence and senescence were identified using microarray in osteosarcoma cell line, U2OS. Besides, it was also noted that certain genes and pathways like NOD pathway was shared by both the growth arrest conditions. A major highlight of the present study was increased expression of number of chemokines and cytokines in both quiescence and senescence. While senescence-associated secretory phenotype (SASP) is well known, the quiescence-associated secretory phenotype (QASP) is relatively unknown and appeared novel in this study. ARID5A, a subunit of SWI/SNF complex was identified as a quiescence associated gene. The endogenous expression of ARID5A increased during serum starved condition of quiescence. Overexpression of ARID5A resulted in more number of cells in G0/G1 phase of cell cycle. Further ARID5A overexpressing cells when subjected to serum starvation showed a pronounced secretory phenotype. Overall, the present work has identified gene expression signatures which can distinguish quiescence from senescence. This article is protected by copyright. All rights reserved.

Item Type: Article
Depositing User: Dr P Divakar
Date Deposited: 22 Oct 2017 18:01
Last Modified: 26 Apr 2018 19:20
URI: http://cdfd.sciencecentral.in/id/eprint/803

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