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Li, S. and Ajimura, M. and Chen, Z. and Liu, J. and Chen, E. and Guo, H. and Vidya, T. and Gangi Reddy, C. and Zhang, J. and Kishino, H. and Abe, H. and Xia, Q. and Arunkumar, K.P. and Mita, K. (2018) A new approach for comprehensively describing heterogametic sex chromosomes. DNA Research, 25 (4). pp. 375-382. ISSN 1340-2838

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Notwithstanding the rapid developments in sequencing techniques, Y and W sex chromosomes have still been mostly excluded from whole genome sequencing projects due to their high repetitive DNA content. Therefore, Y and W chromosomes are poorly described in most species despite their biological importance. Several methods were developed for identifying Y or W-linked sequences among unmapped scaffolds. However, it is not enough to discover functional regions from short unmapped scaffolds. Here, we provide a new and simple strategy based on k-mer comparison for comprehensive analysis of the W chromosome in Bombyx mori. Using this novel method, we effectively assembled de novo 1281 W-derived genome contigs (totaling 1.9 Mbp), and identified 156 W-linked transcript RNAs and 345 W-linked small RNAs. This method will help in the elucidation of mechanisms of sexual development and exploration of W chromosome biological functions, and provide insights into the evolution of sex chromosomes. Moreover, we showed this method can be employed in identifying heterogametic sex chromosomes (W and Y chromosomes) in many other species where genomic information is still scarce.

Item Type: Article
Depositing User: Users 2 not found.
Date Deposited: 07 Apr 2018 17:25
Last Modified: 27 Aug 2018 06:21
URI: http://cdfd.sciencecentral.in/id/eprint/836

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