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Gudla, R. and Konduru, G.V. and Nagarajaram, H.A. and Siddavattam, D. (2019) Organophosphate hydrolase interacts with Ton components and is targeted to the membrane only in the presence of the ExbB/ExbD complex. FEBS Letters, 593 (6). pp. 581-593. ISSN 0014-5793

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Our study aims to investigate the physiological role of organophosphate hydrolase (OPH), hitherto known for its involvement in the degradation of organophosphate insecticides and nerve agents in Sphingobium fuliginis. We find that OPH exists as part of the TonB-dependent Transport (TonBDT) system that is involved in nutrient transport across the bacterial outer membrane. OPH interacts physically with the Ton complex components ExbD and TonB. The surface-exposed arginine residues (R91 and R96) of OPH facilitate its interaction with ExbD. OPH is targeted to the inner membrane of E. coli only when it is co-expressed with either ExbD or the ExbB/ExbD complex. In the absence of ExbD, OPH remains in the cytoplasm. Our findings suggest a role for OPH in outer membrane transport

Item Type: Article
Depositing User: Dr P Divakar
Date Deposited: 27 Feb 2019 07:55
Last Modified: 29 Mar 2019 07:58
URI: http://cdfd.sciencecentral.in/id/eprint/888

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