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Ghosh, D.K. and Shrikondawar, A.N. and Ranjan, Akash (2020) Local structural unfolding at the edge-strands of beta sheets is the molecular basis for instability and aggregation of G85R and G93A mutants of Superoxide dismutase 1. Journal of Biomolecular Structure and Dynamics, 38 (3). pp. 647-653. ISSN 0739-1102

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Dynamic nature of structural segments is a key modulator of protein's intrinsic stability. Mutants of Superoxide dismutase 1 (SOD1) protein, like SOD1G85R and SOD1G93A, adopt misfolded states that undergo aggregation in motor neuron cells. In this study, we had used correlative computational studies to investigate the spatiotemporal flux of structural alterations in SOD1G85R and SOD1G93A that increased the instability and aggregation propensity of these proteins. Molecular dynamics simulation studies showed that the G85R and G93A mutations caused localized transitions of the edge-strands of beta sheets to disordered structures near the mutation regions. Though this structural perturbation did not to alter the conformation of the catalytic zinc and copper binding residues, it could dislocate the electrostatic loop of SOD1G85R. This had rendered the electrostatic loop of SOD1G85R to be incapable of guiding the substrate to catalytic cleft. The beta sheet-to-disorder transitions near the mutations had caused steric clashes in the edge-strand residues, resulting in the loss of several intra-molecular interactions in the mutant SOD1 proteins. These had effected in local structural destabilization and increased aggregation potential in SOD1G85R and SOD1G93A. Mutant SOD1 proteins adopted energetically less favorable states, with some changes in the residue-level conformation entropy and solvent exposed surfaces of the mutation neighboring residues. Collectively, our study demonstrated that the two mutations, G85R and G93A, did not have global effects in changing the SOD1 structure. Instead, the instability-associated aggregation of these mutants arose due to the local structural alterations in the edge-strands of specific beta sheets.

Item Type: Article
Depositing User: Dr P Divakar
Date Deposited: 03 Mar 2019 20:21
Last Modified: 17 Feb 2020 06:13
URI: http://cdfd.sciencecentral.in/id/eprint/889

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