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Reddy, K.D. and Nagaraju, J. and Abraham, E.G. (1999) Genetic characterization of the silkworm Bombyx mori by simple sequence repeat (SSR)-anchored PCR. Heredity, 83 (6). pp. 681-687. ISSN 0018-067X

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Abstract

Thirteen diverse strains of the silkworm Bombyx mori were analysed using the simple sequence repeat anchored polymerase chain reaction (SSR-anchored PCR) or Inter-SSR-PCR (ISSR-PCR). A set of four 5'-anchored and two 3'-anchored repeat primers amplified a total of 239 bands out of which 184 (77%) were polymorphic. The 5'-anchored primers revealed more distinct polymorphic markers than the 3'-anchored primers and the ISSR-PCR method showed greater variability than RAPDs. The strain-specific pattern was shown to be inherited and segregated in a Mendelian fashion. A dendrogram constructed using the UPGMA method revealed two distinct groups, one comprising nondiapausing and one comprising diapausing strains. These results suggest that the ISSR-PCR method is potentially useful for genetic fingerprinting of silkworm genotypes and as a mapping tool in the silkworm.

Item Type: Article
Subjects: Genetics
Depositing User: Dr P Divakar
Date Deposited: 26 May 2015 20:05
Last Modified: 27 May 2015 11:44
URI: http://cdfd.sciencecentral.in/id/eprint/92

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