![[feed]](/style/images/feed-icon-14x14.png){kind=icon}
Raghavendra, V. and Chatterjee, Nirupama and Haldar, Devyani (2020) Cellular environment controls the dynamics of histone H3 lysine 56 acetylation in response to DNA damage in mammalian cells. Journal of Biosciences, 45 (1). p. 19. ISSN 0250-5991
|
Text
J Biosci 45 e19.pdf Download (1512Kb) | Preview |
Abstract
Epigenetic changes play a crucial role in sensing signals and responding to fluctuations in the extracellular environment. How the cellular micro-environment affects DNA damage response signalling in chromatin context is not extensively studied. Histone acetylation is dynamic and very sensitive to changes in the extracellular environment. Existing literature on H3 lysine 56 acetylation (H3K56ac) levels upon DNA damage in mammals presents a conflicting picture. The occurrence of both increased and decreased H3K56ac upon DNA damage in our experiments led us to investigate the role of the micro-environment on H3K56ac. Here, we show that the global levels of H3K56ac increase as cells grow from low density to high density while SIRT1 and SIRT6 expression decrease. Additionally, rising lactic acid levels increase H3K56ac. Our results show that cell density and accumulation of metabolites affect dynamics of H3K56ac in response to DNA damage. Upon DNA damage, H3K56ac increases in low density cells with low initial acetylation, while acetylation decreases in high cell density cells. These results highlight that H3K56ac levels upon DNA damage are dependent on the metabolites in the extracellular milieu which impact chromatin structure by regulating chromatin modifying enzymes. Accumulation of lactic acid at high cell density reflects conditions similar to the tumour micro-environment. As H3K56ac increases in tumours, lactic acid and low pH might alter H3K56ac in tumours, leading to deregulated gene expression, contributing to tumour progression.
| Item Type: | Article |
|---|---|
| Subjects: | Genetics |
| Depositing User: | Users 2 not found. |
| Date Deposited: | 05 Feb 2020 19:55 |
| Last Modified: | 05 Feb 2020 19:58 |
| URI: | http://cdfd.sciencecentral.in/id/eprint/940 |
Actions (login required)
 |
View Item |