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Anupama, K. and Pranathi, K. and Sundaram, R.M. (2020) Assessment of genetic purity of bulked‐seed of rice CMS‐lines using capillary electrophoresis. ELECTROPHORESIS, 41 (20). pp. 1749-1751. ISSN 0173-0835

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Abstract

Genetically pure CMS line (A-line) is essential to generate pure hybrid in order to harness the yield heterosis in rice. Conventionally, seed purity testing is carried by grow-out test (GOT), which has many limitations. Seed purity assessments based on molecular markers, reduces the time required for analysis significantly. However, it is very tedious since at least 200-400 seeds/seedlings are needed to be analyzed individually. An assay based on bulked-seed and molecular markers will be an ideal system. Keeping these points in view, in the present study, a co-dominant mitochondrial marker was used to test the purity of bulked parental line (A-line) seed utilising capillary electrophoresis system in a Genetic analyser. The results indicate that this method is very simple, accurate and can be used to test purity of large number of samples rapidly in a cost effective way compared to GOT and conventional molecular marker analysis.

Item Type: Article
Subjects: Plant Molecular Biology
Depositing User: Dr P. Divakar
Date Deposited: 10 May 2020 21:00
Last Modified: 10 Nov 2020 19:25
URI: http://cdfd.sciencecentral.in/id/eprint/953

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