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Sen, Ranjan and Dasgupta, Dipak (2003) Simple Fluorescence Assays Probing Conformational Changes of Escherichia coli RNA Polymerase During Transcription Initiation. Methods in Enzymology, 370. pp. 598-605. ISSN 0076-6879

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Abstract

As fluorescence signals are indirect measurements of conformational changes, it is important to use these techniques on biochemically characterized RNAP–promoter complexes. If these were to be applied to a new system, it is advisable to perform parallel biochemical assays such as DNA footprinting to confirm the existence of intermediate states.

Item Type: Article
Subjects: Molecular Biology
Depositing User: Dr P Divakar
Date Deposited: 09 Jun 2015 07:26
Last Modified: 09 Jun 2015 07:26
URI: http://cdfd.sciencecentral.in/id/eprint/158

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