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Rahul, G. and Sangeetha, V. and Kumar, Kundan and Thakur, S.S. and Mishra, Krishnaveni and Kaur, Rupinder (2016) Identification of components of the SUMOylation machinery in Candida glabrata: role of the deSUMOylation peptidase CgUlp2 in virulence. Journal of Biological Chemistry, 291 (37). pp. 19573-19589. ISSN 0021-9258

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Abstract

Regulation of protein function by reversible post-translational modification, SUMOylation, is widely conserved in the eukaryotic kingdom. SUMOylation is essential for cell growth, division and adaptation to stress in most organisms including fungi. As these are key factors in determination of fungal virulence, in this study, we have investigated the importance of SUMOylation in the human pathogen, Candida glabrata. We identified the enzymes involved in SUMO conjugation and show that there is strong conservation between S. cerevisiae and C. glabrata. We demonstrate that SUMOylation is an essential process and that adaptation to stress involves changes in global SUMOylation in C. glabrata. Importantly, loss of the deSUMOylating enzyme CgUlp2 leads to highly reduced SUMO protein levels, and impaired growth, sensitivity to multiple stress conditions, reduced adherence to epithelial cells and poor colonization of specific tissues in mice. Our study thus demonstrates a key role for protein SUMOylation in the life cycle and pathobiology of C. glabrata.

Item Type: Article
Depositing User: Dr P Divakar
Date Deposited: 12 Jul 2016 04:41
Last Modified: 13 Oct 2017 08:59
URI: http://cdfd.sciencecentral.in/id/eprint/736

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