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Jain, Sriyans and Gupta, Richa and Sen, Ranjan (2019) Rho-dependent transcription termination in bacteria recycles RNA polymerases stalled at DNA lesions. Nature Communications, 10. p. 1207. ISSN 2041-1723

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Abstract

In bacteria, transcription-coupled repair of DNA lesions initiates after the Mfd protein removes RNA polymerases (RNAPs) stalled at the lesions. The bacterial RNA helicase, Rho, is a transcription termination protein that dislodges the elongation complexes. Here, we show that Rho dislodges the stalled RNAPs at DNA lesions. Strains defective in both Rho and Mfd are susceptible to DNA-damaging agents and are inefficient in repairing or propagating UV-damaged DNA. In vitro transcription assays show that Rho dissociates the stalled elongation complexes at the DNA lesions. We conclude that Rho-dependent termination recycles stalled RNAPs, which might facilitate DNA repair and other DNA-dependent processes essential for bacterial cell survival. We surmise that Rho might compete with, or augment, the Mfd function

Item Type: Article
Subjects: Molecular Biology
Depositing User: Dr P Divakar
Date Deposited: 21 Mar 2019 12:02
Last Modified: 21 Mar 2019 12:02
URI: http://cdfd.sciencecentral.in/id/eprint/890

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