Parine, N.R. and Lakshmi, P. and Kumar, D. and Shaik, J.P. and Alanazi, M. and Pathan, A.A.K. (2015) Development and characterisation of nine polymorphic microsatellite markers for Tephrosia calophylla Bedd. (Fabaceae). Saudi Journal of Biological Sciences, 22 (2). pp. 164-167. ISSN 1319-562X

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Abstract

Tephrosia calophylla Bedd. (Fabaceae) is an endangered tropical plant endemic to southwestern Ghats, India. The objective of this study was to contribute to the characterisation of the diversity of this rare species, which is necessary for its future conservation. Accordingly, microsatellite markers were designed, and their ability to detect polymorphisms was determined. Nine microsatellite markers were developed using genomic libraries, and all of the markers were successfully amplified in 42 individuals. Three to nine alleles per locus were observed, and the heterozygosity of the loci ranged from 0.381 to 0.905. The nine newly developed polymorphic markers recognise a sufficient number of varying loci to perform further studies on the conservation and breeding of this medicinal cultivar.

Item Type:	Article
Uncontrolled Keywords:	Conservation breeding, Endangered medicinal plants, Tephrosia calophylla, Microsatellite markers, Fabaceae
Subjects:	Plant Molecular Biology
Depositing User:	Users 2 not found.
Date Deposited:	16 May 2015 09:55
Last Modified:	19 May 2015 05:15
URI:	http://cdfd.sciencecentral.in/id/eprint/31

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