Gowrishankar, J. and Yamamoto, K. and Subbarayan, P.R. and Ishihama, A. (2003) In vitro properties of RpoS ( S) mutants of Escherichia coli with postulated N-terminal subregion 1.1 or C-terminal region 4 deleted. Journal of Bacteriology, 185 (8). pp. 2673-2679. ISSN 0021-9193

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Abstract

Derivatives of the stationary-phase sigma factor sigma(S) of Escherichia coli lacking either of two conserved domains, the postulated N-terminal subregion 1.1 or the C-terminal region 4, were shown to be competent in vitro for transcription initiation from several sigma(S)-dependent promoters on supercoiled DNA templates. Unlike wild-type sigma(S), however, the deletion derivatives were inactive on relaxed templates. The anomalous slow electrophoretic mobility of sigma(S) on denaturing gels was corrected by deletion of subregion 1.1, suggesting that this domain in sigma(S) may be structurally and functionally analogous to subregion 1.1 of sigma(70), substitutions in which have previously been shown to rectify the anomalous electrophoretic migration of sigma(70) (V. Gopal and D. Chatterji, Eur. J. Biochem. 244:614-618, 1997).

Item Type:	Article
Depositing User:	Users 2 not found.
Date Deposited:	07 Oct 2015 06:06
Last Modified:	07 Oct 2015 06:06
URI:	http://cdfd.sciencecentral.in/id/eprint/543

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