Shashni, R. and Qayyum, M.Z. and Vishalini, V. and Dey, D. and Sen, Ranjan (2014) Redundancy of primary RNA-binding functions of the bacterial transcription terminator Rho. Nucleic Acids Research, 42 (15). pp. 9677-9690. ISSN 0305-1048

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Abstract

The bacterial transcription terminator, Rho, terminates transcription at half of the operons. According to the classical model derived from in vitro assays on a few terminators, Rho is recruited to the transcription elongation complex (EC) by recognizing specific sites (rut) on the nascent RNA. Here, we explored the mode of in vivo recruitment process of Rho. We show that sequence specific recognition of the rut site, in majority of the Rho-dependent terminators, can be compromised to a great extent without seriously affecting the genome-wide termination function as well as the viability of Escherichia coli. These terminators function optimally only through a NusG-assisted recruitment and activation of Rho. Our data also indicate that at these terminators, Rho-EC-bound NusG interaction facilitates the isomerization of Rho into a translocase-competent form by stabilizing the interactions of mRNA with the secondary RNA binding site, thereby overcoming the defects of the primary RNA binding functions

Item Type:	Article
Subjects:	Molecular Biology
Depositing User:	Users 2 not found.
Date Deposited:	16 May 2015 19:56
Last Modified:	28 Nov 2015 19:07
URI:	http://cdfd.sciencecentral.in/id/eprint/40

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